Day 12 of why i'm not on deepworld because of my thesis project:
So four out of the six batches of fly brains were successfully washed and stained, two were lost to the void that is losing an infinitesimally small piece of white tissue on a white surface. Both were replaced though. I should be done with the first set of fly brains this week and then i can finally take a day off!
Also I've discovered the joys and pains of coffee and i will be chugging one (1) raspberry coco coffee each night to stay awake in the lab.
Day 11 of why I'm not on deepworld because of my thesis project:
There comes a time in your life when you realize that you've made a horrible mistake. Today that mistake was setting up not one but SIX batches of flies to be tested tomorrow. That's SIX rounds of testing, dissecting, and 2 hours each of washing and antibody staining.
Day 10 of why I'm not on deepworld because of my thesis project:
Just found out that two weeks worth of brain dissection and antibody staining was for absolutely mcfreakin nothing. The brains got discarded accidentally somewhere in the 292847583 PBST rinses they went through :')
Day 8 of why I'm not on deepworld because of my thesis project: Sometimes you just gotta google how to dissect a fly brain and then frantically email your professor at 8pm what the difference between washing and rinsing is only to spill the last remains of the last dissection you had left all over the floor.
Day 7 of why I'm on deepworld because of thesis project: Testing starts today!!! I'm gonna test to see how well they can climb, and then hopefully i remember how to dissect fly brain for the antibody staining!!!!
Day 5 of why I'm not on deepworld because of my thesis project: One of the batches of flies decieded they wanted to become celibate monks, therefore, Ive got no fruit fly subjects to test on. Another batch decided they've had enough of living in a test tube with a cotton ball stopper and liked sticking themselves to the wall better. Its been 2 weeks, my sanity is slowly fading.
Day 4 of why I'm not on deepworld because of my thesis: Snowpocalypse put me 2 days behind on work. I'm supposed to start testing tomorrow but none of my supplies are ready. No one has checked on the flies. Send help :(
You ever accidentally drop your cat 90 meters and then try to raise it back up except the cat keeps falling because someone decided to use dirt blocks and a shovel instead of real boy stone blocks and each time the cat falls it takes another year off your life and oh god why did I open the cat crate right next to a hole in the wall why
YEAH SEE THATS WHAT HAPPENED AFTER WE MADE A DIRT TOWER TO GET IT BACK UP THERE.
We had 5 people including myself trying to get to this cat up to the top of an apartment building and each time someone shoveled the wrong block down the cat went. So stressful 😰
Day 2 of the reason I'm not on deepworld because of my thesis project: Had to put down two flies because one decided to stick its leg where it didnt belong. Back to the CO2 pad I go, time to look for EVEN MORE replacements!!!!